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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM126A Recombinant antibody (83440-2-RR)

    WB analysis using 83440-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83440-2-RR (TMEM126A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMEM126A Recombinant antibody (83440-2-RR)

    IF Staining of HeLa using 83440-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM126A antibody (83440-2-RR, Clone: 240425A11 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using TMEM126A Recombinant antibody (83440-2-RR)

    FC experiment of HeLa using 83440-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TMEM126A Recombinant antibody (83440-2-RR, Clone:240425A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83440-2-RR

    Affinity and Kinetic Characterization of 83440-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83440-2-RR against Human TMEM126A were performed. The affinity constant is 0.589 nM.

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TMEM126A Recombinant antibody

TMEM126A Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83440-2-RR
Clone No.240425A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TMEM126, TMEM, OPA7, 240425A11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inT-47D cells, HeLa cells, MCF-7 cells, U-251 cells, U2OS cells, human placenta tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83440-2-RR targets TMEM126A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM126A fusion protein Ag14705 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 126A
Calculated molecular weight 195 aa, 22 kDa
Observed molecular weight20-25 kDa
GenBank accession numberBC007875
Gene Symbol TMEM126A
Gene ID (NCBI) 84233
RRIDAB_3671081
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H061
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM126A is a mitochondrial inner membrane protein that is enriched in the cristae. TMEM126A is necessary for Complex I assembly or stability.Complex I (CI) is the largest enzyme of the mitochondrial respiratory chain, and its defects are the main cause of mitochondrial disease.

Protocols

Product Specific Protocols
WB protocol for TMEM126A antibody 83440-2-RRDownload protocol
IF protocol for TMEM126A antibody 83440-2-RRDownload protocol
FC protocol for TMEM126A antibody 83440-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols