TMEM127 Polyclonal antibody
TMEM127 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 23142-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer tissue, human heart tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
23142-1-AP targets TMEM127 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TMEM127 fusion protein Ag19472 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 127 |
| Calculated molecular weight | 238 aa, 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC039892 |
| Gene symbol | TMEM127 |
| Gene ID (NCBI) | 55654 |
| RRID | AB_2879217 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM127 antibody 23142-1-AP | Download protocol |
| IF protocol for TMEM127 antibody 23142-1-AP | Download protocol |
| IP protocol for TMEM127 antibody 23142-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression | ||
Tissue Cell Exosomes from adipose-derived stem cells overexpressing microRNA-671-3p promote fat graft angiogenesis and adipogenic differentiation |
