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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM132E Recombinant antibody

TMEM132E Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230525E12

Cat no : 82983-2-RR

Synonyms

transmembrane protein 132E, 230525E12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM132E Recombinant antibody (82983-2-RR)

    WB analysis using 82983-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82983-2-RR (TMEM132E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82983-2-RR

    Affinity and Kinetic Characterization of 82983-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82983-2-RR against Human TMEM132E were performed. The affinity constant is 0.254 nM.

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Tested Applications

Positive WB detected inMCF-7 cells, mouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82983-2-RR targets TMEM132E in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM132E fusion protein Ag26772 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 132E
Calculated molecular weight 116 kDa
Observed molecular weight100-110 kDa
GenBank accession numberNM_001304438
Gene symbol TMEM132E
Gene ID (NCBI) 124842
RRIDAB_3670733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transmembrane protein 132E (TMEM132E) is a member of the TMEM132 family which also include TMEM132A, TMEM132B, TMEM132C, TMEM132D (PMID: 29088312). The gene of TMEM132E is located within the DFNB99 locus on chromosome 17q12 and is highly expressed in the inner ear and other tissues including brain, kidney, lung, liver, spleen, heart, small intestine, colon, thymus, and stomach (PMID: 31656313). A variant of TMEM132E has been associated with autosomal-recessive nonsyndromic hearing loss DFNB99 (PMID: 25331638).

Protocols

Product Specific Protocols
WB protocol for TMEM132E antibody 82983-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols