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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using TMEM165 Monoclonal antibody (68584-1-Ig)

    WB analysis of LNCaP using 68584-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TMEM165 Monoclonal antibody (68584-1-Ig)

    WB analysis of K-562 using 68584-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-251 cells using TMEM165 Monoclonal antibody (68584-1-Ig)

    WB analysis of U-251 using 68584-1-Ig

    U-251 cells were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TMEM165 Monoclonal antibody (68584-1-Ig)

    WB analysis using 68584-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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TMEM165 Monoclonal antibody

TMEM165 Monoclonal Antibody for WB, ELISA
Cat No. 68584-1-Ig
Clone No.3B11C9

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

TMEM165, TMPT27, TPARL, transmembrane protein 165, Transmembrane protein PT27, Transmembrane protein TPARL

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSaos-2 cells, U-251 cells, LNCaP cells, K-562 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68584-1-Ig targets TMEM165 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMEM165 fusion protein Ag14872 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 165
Calculated molecular weight 324 aa, 35 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC003545
Gene Symbol TMEM165
Gene ID (NCBI) 55858
RRIDAB_3085284
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9HC07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM165 antibody 68584-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols