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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM165 Recombinant antibody

TMEM165 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230517B1

Cat no : 83146-1-RR

Synonyms

Transmembrane protein PT27, Transmembrane protein 165, TPARL, TMPT27, Putative divalent cation/proton antiporter TMEM165

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMEM165 Recombinant antibody (83146-1-RR)

    IF Staining of HepG2 using 83146-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM165 antibody (83146-1-RR, Clone: 230517B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using TMEM165 Recombinant antibody (83146-1-RR)

    FC experiment of HepG2 using 83146-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM165 Recombinant antibody (83146-1-RR, Clone:230517B1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83146-1-RR

    Affinity and Kinetic Characterization of 83146-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83146-1-RR against Human TMEM165 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83146-1-RR targets TMEM165 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM165 fusion protein Ag14362 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 165
Calculated molecular weight 324 aa, 35 kDa
GenBank accession numberBC003545
Gene symbol TMEM165
Gene ID (NCBI) 55858
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT), a member of methyltransferase superfamily, is an enzyme involved in the normal metabolic inactivation of thiopurine drugs such as azathioprine, 6-mercaptopurine and 6-thioguanine. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Defects in the TPMT gene leads to decreased methylation and decreased inactivation of 6MP leading to enhanced bone marrow toxicity which may cause myelosuppression, anemia, bleeding tendency, leukopenia and infection.

Protocols

Product Specific Protocols
IF protocol for TMEM165 antibody 83146-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols