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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM173/STING Recombinant antibody (80165-1-RR)

    WB analysis using 80165-1-RR (same clone as 80165-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80165-1-RR (TMEM173/STING antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using TMEM173/STING Recombinant antibody (80165-1-RR)

    IP experiment of HeLa using 80165-1-RR (same clone as 80165-1-PBS)

    IP result of anti-TMEM173/STING (IP: 80165-1-RR, 4ug; Detection: 80165-1-RR 1:8000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TMEM173/STING Recombinant antibody (80165-1-RR)

    IHC staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TMEM173/STING Recombinant antibody (80165-1-RR)

    IHC staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TMEM173/STING Recombinant antibody (80165-1-RR)

    IHC staining of human colon using 80165-1-RR (same clone as 80165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM173/STING Recombinant antibody (80165-1-RR)

    IHC staining of human liver using 80165-1-RR (same clone as 80165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM173/STING Recombinant antibody (80165-1-RR)

    IHC staining of human liver using 80165-1-RR (same clone as 80165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using TMEM173/STING Recombinant antibody (80165-1-RR)

    IF Staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMEM173/STING antibody (80165-1-RR, Clone: 7K7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using TMEM173/STING Recombinant antibody (80165-1-RR)

    IF Staining of THP-1 using 80165-1-RR (same clone as 80165-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TMEM173/STING antibody (80165-1-RR, Clone: 7K7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80165-1-RR

    Affinity and Kinetic Characterization of 80165-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80165-1-RR against Human TMEM173/STING were performed. The affinity constant is 0.776 nM.

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TMEM173/STING Recombinant antibody, PBS Only

TMEM173/STING Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 80165-1-PBS
Clone No.7K7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

STING, NET23, MPYS, MITA, ERIS

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80165-1-PBS targets TMEM173/STING in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM173/STING fusion protein Ag13921 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 173
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight37 kDa
GenBank accession numberBC047779
Gene Symbol STING
Gene ID (NCBI) 340061
RRIDAB_2918870
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86WV6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.