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  • Featured Product
  • KD/KO Validated

TMEM175 Polyclonal antibody, PBS Only

TMEM175 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19925-1-PBS

Synonyms

Transmembrane protein 175, hTMEM175

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM175 Polyclonal antibody (19925-1-AP)

    WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    Boiled and unboiled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM175 Polyclonal antibody (19925-1-AP)

    WB analysis using 19925-1-AP (same clone as 19925-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19925-1-AP (TMEM175 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM175 Polyclonal antibody (19925-1-AP)

    IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM175 Polyclonal antibody (19925-1-AP)

    IHC staining of mouse brain using 19925-1-AP (same clone as 19925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19925-1-AP (TMEM175 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19925-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19925-1-PBS targets TMEM175 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM175 fusion protein Ag13890 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 175
Calculated molecular weight 504 aa, 56 kDa
Observed molecular weight41-45 kDa, 54-60 kDa
GenBank accession numberBC005158
Gene symbol TMEM175
Gene ID (NCBI) 84286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.