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  • Featured Product
  • KD/KO Validated

TMEM175 Monoclonal antibody, PBS Only

TMEM175 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E7E1

Cat no : 68172-1-PBS

Synonyms

Transmembrane protein 175, hTMEM175, 1E7E1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TMEM175 Monoclonal antibody (68172-1-Ig)

    WB analysis of HepG2 using 68172-1-Ig (same clone as 68172-1-PBS)

    WB result of TMEM175 antibody (68172-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM175 transfected HepG2 cells. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM175 Monoclonal antibody (68172-1-Ig)

    WB analysis using 68172-1-Ig (same clone as 68172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using TMEM175 Monoclonal antibody (68172-1-Ig)

    WB analysis of pig liver using 68172-1-Ig (same clone as 68172-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM175 Monoclonal antibody (68172-1-Ig)

    WB analysis using 68172-1-Ig (same clone as 68172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68172-1-Ig (TMEM175 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68172-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68172-1-PBS targets TMEM175 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMEM175 fusion protein Ag13890 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 175
Calculated molecular weight 504 aa, 56 kDa
Observed molecular weight55-60 kDa
GenBank accession numberBC005158
Gene symbol TMEM175
Gene ID (NCBI) 84286
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability.