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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM176A Polyclonal antibody, PBS Only

TMEM176A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20378-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using TMEM176A Polyclonal antibody (20378-1-AP)

    WB analysis of fetal human brain using 20378-1-AP (same clone as 20378-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20378-1-AP (TMEM176A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM176A Polyclonal antibody (20378-1-AP)

    IHC staining of mouse brain using 20378-1-AP (same clone as 20378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM176A Polyclonal antibody (20378-1-AP)

    IHC staining of mouse brain using 20378-1-AP (same clone as 20378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM176A Polyclonal antibody (20378-1-AP)

    IHC staining of human liver using 20378-1-AP (same clone as 20378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM176A Polyclonal antibody (20378-1-AP)

    IHC staining of human liver using 20378-1-AP (same clone as 20378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20378-1-AP (TMEM176A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TMEM176A Polyclonal antibody (20378-1-AP)

    IF Staining of HeLa using 20378-1-AP (same clone as 20378-1-PBS)

    Immunofluorescent analysis of Hela cells, using TMEM176A antibody 20378-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20378-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20378-1-PBS targets TMEM176A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM176A fusion protein Ag14194 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 176A
Calculated molecular weight 235 aa, 26 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC008303
Gene symbol TMEM176A
Gene ID (NCBI) 55365
RRIDAB_10697661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.