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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM184B Polyclonal antibody (28031-1-AP)

    IHC staining of mouse brain using 28031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM184B Polyclonal antibody (28031-1-AP)

    IHC staining of mouse brain using 28031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using TMEM184B Polyclonal antibody (28031-1-AP)

    IHC staining of mouse cerebellum using 28031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using TMEM184B Polyclonal antibody (28031-1-AP)

    IHC staining of mouse cerebellum using 28031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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TMEM184B Polyclonal antibody

TMEM184B Polyclonal Antibody for IHC, ELISA
Cat No. 28031-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, ELISA

Synonyms

C22orf5, DKFZp586A1024, DKFZp686F07174, DKFZp686N1150, FM08, HS5O6A, TMEM184B, transmembrane protein 184B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28031-1-AP targets TMEM184B in IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM184B fusion protein Ag27704 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 184B
GenBank accession numberBC015489
Gene Symbol TMEM184B
Gene ID (NCBI) 25829
RRIDAB_3086023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y519
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transmembrane protein 184B (TMEM184B) is a seven-pass transmembrane protein highly expressed in the nervous system and is thought to play a role in neuronal excitability, synaptic structure, and expression of key developmental and adult pathways involved in neuronal differentiation (PMID: 27122027; 37730546). TMEM184B may activate the MAP kinase signaling pathway (PMID: 12761501). TMEM184B has also been reported to be necessary for interleukin-31-induced itch, promoting adult somatosensation through developmental Wnt signaling and promotion of proper pruriceptive gene expression (PMID: 34629389).

Protocols

Product Specific Protocols
IHC protocol for TMEM184B antibody 28031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols