Validation Data Gallery

Filter: AllWBIHC

WB analysis using 28263-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28263-1-AP (TMEM192 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 28263-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 28263-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 28263-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 28263-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 28263-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 28263-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 28263-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 28263-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 28263-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 28263-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM192 Polyclonal antibody

TMEM192 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 28263-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

FLJ38482, TMEM192, transmembrane protein 192

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, U2OS cells
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, human prostate cancer tissue, mouse cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 1 publications below

Product Information

28263-1-AP targets TMEM192 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity	Human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TMEM192 fusion protein Ag27576 相同性解析による交差性が予測される生物種
Full Name	transmembrane protein 192
Observed molecular weight	30 kDa
GenBank accession number	BC036301
Gene Symbol	TMEM192
Gene ID (NCBI)	201931
RRID	AB_2881099
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IY95
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transmembrane protein 192 (TMEM192) has been previously identified in proteomic analyses of lysosomal membranes.TMEM192 shows a wide expression pattern in mouse tissues. Interestingly, TMEM192 was found to be highly expressed in tumor cell lines, while it was not expressed or detected at low levels in normal cell lines(PMID: 22736246). TMEM192 can be detected as 30 kDa.

Protocols

Product Specific Protocols
WB protocol for TMEM192 antibody 28263-1-AP	Download protocol
IHC protocol for TMEM192 antibody 28263-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Mol Ther Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase Authors - Yang Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate