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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM199 Polyclonal antibody, PBS Only

TMEM199 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25489-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using TMEM199 Polyclonal antibody (25489-1-AP)

    WB analysis of NIH/3T3 using 25489-1-AP (same clone as 25489-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25489-1-AP (TMEM199 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25489-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM199 Polyclonal antibody (25489-1-AP)

    IHC staining of human breast cancer using 25489-1-AP (same clone as 25489-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25489-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM199 Polyclonal antibody (25489-1-AP)

    IHC staining of human breast cancer using 25489-1-AP (same clone as 25489-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25489-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TMEM199 Polyclonal antibody (25489-1-AP)

    IHC staining of human stomach cancer using 25489-1-AP (same clone as 25489-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25489-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TMEM199 Polyclonal antibody (25489-1-AP)

    IHC staining of human stomach cancer using 25489-1-AP (same clone as 25489-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25489-1-AP (TMEM199 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25489-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25489-1-PBS targets TMEM199 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM199 fusion protein Ag22009 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 199
Calculated molecular weight 208 aa, 23 kDa
Observed molecular weight 18-23 kDa
GenBank accession numberBC033113
Gene symbol TMEM199
Gene ID (NCBI) 147007
RRIDAB_2880101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TMEM199, also named as C17orf32, is a component of the complex sense antisense architecture (CSAGA).