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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM200C Polyclonal antibody

TMEM200C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28596-1-AP

Synonyms

TTMA, Transmembrane protein TTMA, Transmembrane protein 200C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM200C Polyclonal antibody (28596-1-AP)

    WB analysis using 28596-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28596-1-AP (TMEM200C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using TMEM200C Polyclonal antibody (28596-1-AP)

    IHC staining of mouse kidney using 28596-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28596-1-AP (TMEM200C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using TMEM200C Polyclonal antibody (28596-1-AP)

    IHC staining of mouse kidney using 28596-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28596-1-AP (TMEM200C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28596-1-AP targets TMEM200C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM200C fusion protein Ag29577 相同性解析による交差性が予測される生物種
Full Name two transmembrane domain family member A
Calculated molecular weight 64 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_001080209
Gene symbol TTMA
Gene ID (NCBI) 645369
RRIDAB_3669664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM200C (Transmembrane protein 200C) may be an integral component of the membrane.

Protocols

Product Specific Protocols
WB protocol for TMEM200C antibody 28596-1-APDownload protocol
IHC protocol for TMEM200C antibody 28596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols