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  • Featured Product
  • KD/KO Validated

TMEM25 Polyclonal antibody, PBS Only

TMEM25 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24361-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM25 Polyclonal antibody (24361-1-AP)

    WB analysis using 24361-1-AP (same clone as 24361-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24361-1-AP (TMEM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24361-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM25 Polyclonal antibody (24361-1-AP)

    IHC staining of human liver using 24361-1-AP (same clone as 24361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24361-1-AP (TMEM25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24361-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24361-1-PBS targets TMEM25 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM25 fusion protein Ag19964 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 25
Calculated molecular weight 366 aa, 39 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC051841
Gene symbol TMEM25
Gene ID (NCBI) 84866
RRIDAB_2879507
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TMEM25 is a novel member of the immunoglobulin superfamily. BC042896 and AY358919 cDNAs are derived from human TMEM25 gene. TMEM25 isoform 1 (BC042896), consisting of exons 1-9, encodes a 366-aa transmembrane protein. TMEM25 isoform 2 (AY358919), consisting of exons 1-4 and 6-9, encodes a 322-aa secreted protein (PMID:15254712). Human TMEM25 gene are found to encode transmembrane-type as well as secreted-type proteins due to alternative splicing of exon-skipping type. TMEM25 mRNA is expressed in brain, including cerebellar cortex and hippocampus, as well as in neuroblastoma, brain tumors, and gastric cancer. TMEM25 is a target of pharmacogenomics in the field of oncology and regenerative medicine. TMEM25 mRNAs may be useful members of a panel of favorable prognostic and predictive markers for breast cancer( PMID:19776672).