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  • Featured Product
  • KD/KO Validated

TMEM38B Polyclonal antibody

TMEM38B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19919-1-AP

Synonyms

Trimeric intracellular cation channel type B, TRICB, TRIC B, D4Ertd89e, C9orf87

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM38B Polyclonal antibody (19919-1-AP)

    WB analysis using 19919-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19919-1-AP (TMEM38B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TMEM38B Polyclonal antibody (19919-1-AP)

    WB analysis of MCF-7 using 19919-1-AP

    WB result of TMEM38B antibody (19919-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM38B transfected MCF-7 cells.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of mouse testis using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of mouse testis using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of human kidney using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of human kidney using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMEM38B Polyclonal antibody (19919-1-AP)

    IF Staining of HepG2 using 19919-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM38B antibody (19919-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inmouse testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19919-1-AP targets TMEM38B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM38B fusion protein Ag13835 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 38B
Calculated molecular weight 291 aa, 33 kDa
Observed molecular weight28 kDa
GenBank accession numberBC000049
Gene symbol TMEM38B
Gene ID (NCBI) 55151
RRIDAB_2878623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM38B encodes endoplasmic reticulum membrane monovalent cation-specific channel which is involved in the release of calcium (Ca2+) from intracellular stores. TMEM38B, also known as TRIC-B, is one of the two trimeric intracellular cation (TRIC) channels. TRIC-B deficiency causes bone disease due to defective Ca2+ release and signaling in the bone cells. (PMID: 34036147, PMID: 39385871)

Protocols

Product Specific Protocols
WB protocol for TMEM38B antibody 19919-1-APDownload protocol
IHC protocol for TMEM38B antibody 19919-1-APDownload protocol
IF protocol for TMEM38B antibody 19919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols