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KD/KO Validated

TMEM41B Polyclonal antibody

Name: TMEM41B Polyclonal antibody
Brand: Proteintech
SKU: 29270-1-AP

TMEM41B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 29270-1-AP

Synonyms

Transmembrane protein 41B, KIAA0033

Validation Data Gallery

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AllWBIHC

WB analysis of HeLa using 29270-1-AP
WB result of TMEM41B antibody (29270-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM41B transfected HeLa cells.
WB analysis using 29270-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 29270-1-AP (TMEM41B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of mouse lung using 29270-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 29270-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 29270-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 29270-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 29270-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 29270-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 29270-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, HeLa cells
Positive IHC detected in	mouse lung tissue, mouse testis tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

29270-1-AP targets TMEM41B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TMEM41B fusion protein Ag30810 相同性解析による交差性が予測される生物種
Full Name	transmembrane protein 41B
Calculated molecular weight	32 kDa
Observed molecular weight	22-28 kDa
GenBank accession number	BC035034
Gene symbol	TMEM41B
Gene ID (NCBI)	440026
RRID	AB_2918264
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transmembrane protein 41B (TMEM41B), also known as Stasimon, is a major target of survival motor neuron (SMN)-dependent U12 splicing and has been identified as a protein required for motor circuit function (PMID: 23063131). TMEM41B localizes to the endoplasmic reticulum. It is involved in autophagosome biogenesis and lipid mobilization (PMID: 30126924). TMEM41B has been shown to be essential for mouse embryonic development (PMID: 30352685). Additionally, genome-scale CRISPR knockout screens reveal that TMEM41B is required for infection of flavivirus and coronavirus, including SARS-CoV-2 (PMID: 33052348; 33052332). Alternative splicing results in three transcript variants encoding distinct isoforms.

Protocols

Product Specific Protocols
WB protocol for TMEM41B antibody 29270-1-AP	Download protocol
IHC protocol for TMEM41B antibody 29270-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. Authors - Dong Huang KO Validated View Article
mouse	IHC	Biomedicines Comparative Proteomic Analysis of tPVAT during Ang II Infusion. Authors - Xiuying Liang View Article
human	WB	STAR Protoc In vitro and in vivo assay of the ER lipid scramblase TMEM41B. Authors - Lingzhi Wu KO Validated View Article
mouse,pig	WB,IF	J Virol TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication Authors - Xiu-Qing Li KD Validated View Article
human	WB	Nature PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection Authors - Dijin Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM41B Polyclonal antibody

TMEM41B Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 29270-1-AP

WB analysis using 29270-1-AP

IHC staining of mouse lung using 29270-1-AP

IHC staining of mouse lung using 29270-1-AP

IHC staining of mouse testis using 29270-1-AP

IHC staining of mouse testis using 29270-1-AP

IHC staining of human lung using 29270-1-AP

IHC staining of human lung using 29270-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

In vitro and in vivo assay of the ER lipid scramblase TMEM41B.

TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication

PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection