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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM41B Recombinant antibody (84510-4-RR)

    WB analysis using 84510-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84510-4-RR (TMEM41B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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TMEM41B Recombinant antibody

TMEM41B Recombinant Antibody for WB, ELISA
Cat No. 84510-4-RR
Clone No.242017C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Transmembrane protein 41B, KIAA0033, 242017C11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells, A2780 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84510-4-RR targets TMEM41B in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM41B fusion protein Ag30810 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 41B
Calculated molecular weight32 kDa
Observed molecular weight25-28 kDa
GenBank accession numberBC035034
Gene Symbol TMEM41B
Gene ID (NCBI) 440026
RRIDAB_3672019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5BJD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transmembrane protein 41B (TMEM41B), also known as Stasimon, is a major target of survival motor neuron (SMN)-dependent U12 splicing and has been identified as a protein required for motor circuit function (PMID: 23063131). TMEM41B localizes to the endoplasmic reticulum. It is involved in autophagosome biogenesis and lipid mobilization (PMID: 30126924). TMEM41B has been shown to be essential for mouse embryonic development (PMID: 30352685). Additionally, genome-scale CRISPR knockout screens reveal that TMEM41B is required for infection of flavivirus and coronavirus, including SARS-CoV-2 (PMID: 33052348; 33052332). Alternative splicing results in three transcript variants encoding distinct isoforms.

Protocols

Product Specific Protocols
WB protocol for TMEM41B antibody 84510-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols