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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM45A Polyclonal antibody (27534-1-AP)

    WB analysis using 27534-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27534-1-AP (TMEM45A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TMEM45A Polyclonal antibody (27534-1-AP)

    WB analysis using 27534-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27534-1-AP (TMEM45A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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TMEM45A Polyclonal antibody

TMEM45A Polyclonal Antibody for WB, ELISA
Cat No. 27534-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Dermal papilla-derived protein 7, DERP7, DNA polymerase-transactivated protein 4, DNAPTP4, Transmembrane protein 45A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA-253 cells, A431 cells, MDA-MB-231 cells, SKOV-3 cells, HH cells, HFF cells, HaCaT cells, MJ cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27534-1-AP targets TMEM45A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM45A fusion protein Ag24392 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 45A
Observed molecular weight70-75 kDa
GenBank accession numberBC040355
Gene Symbol TMEM45A
Gene ID (NCBI) 55076
RRIDAB_3669609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM45A, also known as DERP7, DNAPTP4, or FLJ10134, is a transmembrane protein belonging to the TMEM family. TMEM45A is highly expressed in keratinocytes and its expression correlates with keratinization, suggesting a role in normal epidermal physiology(PMID: 22954140; PMID: 24689342). TMEM45A promotes cell proliferation, migration, and invasion in glioblastoma cells, favors epithelial-mesenchymal transition (EMT) in colorectal cancer and promotes cell proliferation by favoring the G1/S cell cycle transition in ovarian cancer cells (PMID: 37936608). The predicted molecular weight of TMEM45A is 32 kDa, and 66-98 kDa has been reported in the literature(PMID: 22954140).

Protocols

Product Specific Protocols
WB protocol for TMEM45A antibody 27534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols