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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM53 Recombinant antibody, PBS Only

TMEM53 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240511C3

Cat no : 83554-3-PBS

Synonyms

Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TMEM53 Recombinant antibody (83554-3-RR)

    IF Staining of A549 using 83554-3-RR (same clone as 83554-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TMEM53 Recombinant antibody (83554-3-RR)

    IF Staining of A549 using 83554-3-RR (same clone as 83554-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using TMEM53 Recombinant antibody (83554-3-RR)

    FC experiment of A549 using 83554-3-RR (same clone as 83554-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug TMEM53 Recombinant antibody (83554-3-RR, Clone:240511C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83554-3-RR

    Affinity and Kinetic Characterization of 83554-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83554-3-RR against Human TMEM53 were performed. The affinity constant is 44.5 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83554-3-PBS is TMEM53 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM53 fusion protein Ag21321 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 53
Calculated molecular weight 277 aa, 32 kDa
GenBank accession numberBC064520
Gene symbol TMEM53
Gene ID (NCBI) 79639
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.