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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMEM59 Recombinant antibody (83656-3-RR)

    IF Staining of HepG2 using 83656-3-RR (same clone as 83656-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TMEM59 antibody (83656-3-RR, Clone: 240457A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83656-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using TMEM59 Recombinant antibody (83656-3-RR)

    FC experiment of HepG2 using 83656-3-RR (same clone as 83656-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM59 Recombinant antibody (83656-3-RR, Clone:240457A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83656-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83656-3-RR

    Affinity and Kinetic Characterization of 83656-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83656-3-RR against Human TMEM59 were performed. The affinity constant is below 1 pM.

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TMEM59 Recombinant antibody, PBS Only

TMEM59 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83656-3-PBS
Clone No.240457A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

UNQ169/PRO195, Liver membrane-bound protein, HSPC001, C1orf8, 240457A1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83656-3-PBS targets TMEM59 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM59 fusion protein Ag1968 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 59
Calculated molecular weight 36 kDa
GenBank accession numberBC016374
Gene Symbol TMEM59
Gene ID (NCBI) 9528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BXS4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TMEM59 is a regulator of autophagy in response to bacterial infection. Overexpression of this protein has been found to promote apoptosis in a glioma cell line. Alternative splicing of TMEM59 results in multiple transcript variants.