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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM61 Polyclonal antibody

TMEM61 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 20997-1-AP

Synonyms

TMEM61, transmembrane protein 61

Validation Data Gallery

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  • Western Blot (WB) analysis of Transfected HEK-293 cells using TMEM61 Polyclonal antibody (20997-1-AP)

    WB analysis of Transfected HEK-293 using 20997-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 20997-1-AP (TMEM61 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TMEM61 Polyclonal antibody (20997-1-AP)

    IHC staining of human kidney using 20997-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20997-1-AP (TMEM61 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TMEM61 Polyclonal antibody (20997-1-AP)

    IHC staining of human kidney using 20997-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20997-1-AP (TMEM61 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20997-1-AP is TMEM61 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM61 fusion protein Ag15044 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 61
Calculated molecular weight 210 aa, 22 kDa
GenBank accession numberBC029775
Gene symbol TMEM61
Gene ID (NCBI) 199964
RRIDAB_2878786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM61 antibody 20997-1-APDownload protocol
IHC protocol for TMEM61 antibody 20997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols