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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM63B Polyclonal antibody (24226-1-AP)

    WB analysis using 24226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24226-1-AP (TMEM63B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TMEM63B Polyclonal antibody (24226-1-AP)

    WB analysis using 24226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24226-1-AP (TMEM63B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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TMEM63B Polyclonal antibody

TMEM63B Polyclonal Antibody for WB, ELISA
Cat No. 24226-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Transmembrane protein 63B, CSC1-like protein 2, C6orf110

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24226-1-AP targets TMEM63B in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM63B fusion protein Ag21573 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 63B
Calculated molecular weight 832 aa, 95 kDa
Observed molecular weight95 kDa
GenBank accession numberBC136769
Gene Symbol TMEM63B
Gene ID (NCBI) 55362
RRIDAB_3085727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ5T3F8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM63B, also known as C6orf110, belongs to the TMEM63 family. TMEM63B is a mechanosensitive cation channel activated by hypoosmotic stress and mechanic stimulation. Genetic deletion of TMEM63B is a cause of necroptosis of outer hair cells (OHCs) and progressive hearing loss (PMID: 32375046).

Protocols

Product Specific Protocols
WB protocol for TMEM63B antibody 24226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols