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  • Featured Product
  • KD/KO Validated

TMEM65 Polyclonal antibody, PBS Only

TMEM65 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21913-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM65 Polyclonal antibody (21913-1-AP)

    WB analysis using 21913-1-AP (same clone as 21913-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM65 Polyclonal antibody (21913-1-AP)

    WB analysis using 21913-1-AP (same clone as 21913-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TMEM65 Polyclonal antibody (21913-1-AP)

    WB analysis of HeLa using 21913-1-AP (same clone as 21913-1-PBS)

    WB result of TMEM65 antibody (21913-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM65 transfected HeLa cells. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TMEM65 Polyclonal antibody (21913-1-AP)

    IHC staining of human colon cancer using 21913-1-AP (same clone as 21913-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TMEM65 Polyclonal antibody (21913-1-AP)

    IHC staining of human colon cancer using 21913-1-AP (same clone as 21913-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using TMEM65 Polyclonal antibody (21913-1-AP)

    IF Staining of NIH/3T3 using 21913-1-AP (same clone as 21913-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Huh-7 cells using TMEM65 Polyclonal antibody (21913-1-AP)

    IF Staining of Huh-7 using 21913-1-AP (same clone as 21913-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Huh-7 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21913-1-PBS targets TMEM65 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM65 fusion protein Ag15509 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 65
Calculated molecular weight 240 aa, 26 kDa
Observed molecular weight20-25 kDa
GenBank accession numberBC032396
Gene symbol TMEM65
Gene ID (NCBI) 157378
RRIDAB_2918075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.