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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using TMEM74 Monoclonal antibody (67773-1-Ig)

    WB analysis of LNCaP using 67773-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using TMEM74 Monoclonal antibody (67773-1-Ig)

    WB analysis of L02 using 67773-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TMEM74 Monoclonal antibody (67773-1-Ig)

    WB analysis of HEK-293 using 67773-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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TMEM74 Monoclonal antibody

TMEM74 Monoclonal Antibody for WB, ELISA
Cat No. 67773-1-Ig
Clone No.2H1B7

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

FLJ30668, TMEM74, transmembrane protein 74

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, L02 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67773-1-Ig targets TMEM74 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMEM74 fusion protein Ag22359 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 74
Calculated molecular weight 305 aa, 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC030710
Gene Symbol TMEM74
Gene ID (NCBI) 157753
RRIDAB_2918538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96NL1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Transmembrane protein 74 (TMEM74), plays an essential role in autophagy during cell starvation and other stress conditions. TMEM74 is localized to the lysosome and autophagosome. It has been rreported that TMEM74-induced autophagy may be associated with PI3K signal transduction. (PMID: 19029833, 18294959)

Protocols

Product Specific Protocols
WB protocol for TMEM74 antibody 67773-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols