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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TMEM77 Polyclonal antibody (23182-1-AP)

    WB analysis of K-562 using 23182-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 23182-1-AP (TMEM77 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TMEM77 Polyclonal antibody (23182-1-AP)

    IHC staining of human ovary tumor using 23182-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23182-1-AP (TMEM77 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TMEM77 Polyclonal antibody (23182-1-AP)

    IHC staining of human ovary tumor using 23182-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23182-1-AP (TMEM77 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TMEM77 Polyclonal antibody (23182-1-AP)

    IHC staining of human heart using 23182-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23182-1-AP (TMEM77 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TMEM77 Polyclonal antibody (23182-1-AP)

    IHC staining of human heart using 23182-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23182-1-AP (TMEM77 Antibody) at dilution of 1:50 (under 40x lens).

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TMEM77 Polyclonal antibody

TMEM77 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23182-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

DRAM2, PRO180, RP5 1180E21.1, TMEM77, transmembrane protein 77, UNQ154/PRO180, WWFQ154

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman ovary tumor tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23182-1-AP targets TMEM77 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM77 fusion protein Ag19711 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 77
Calculated molecular weight 266 aa, 30 kDa
Observed molecular weight 30 kDa, 60 kDa, 70 kDa
GenBank accession numberBC047025
Gene Symbol TMEM77
Gene ID (NCBI) 128338
RRIDAB_2879225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ6UX65
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM77 antibody 23182-1-APDownload protocol
IHC protocol for TMEM77 antibody 23182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols