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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM87A Polyclonal antibody

TMEM87A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25091-1-AP

Synonyms

Transmembrane protein 87A, TM87A, PSEC0094, Elkin1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM87A Polyclonal antibody (25091-1-AP)

    WB analysis using 25091-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using TMEM87A Polyclonal antibody (25091-1-AP)

    WB analysis of MDA-MB-453s using 25091-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TMEM87A Polyclonal antibody (25091-1-AP)

    WB analysis of PC-3 using 25091-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM87A Polyclonal antibody (25091-1-AP)

    IHC staining of human breast cancer using 25091-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM87A Polyclonal antibody (25091-1-AP)

    IHC staining of human breast cancer using 25091-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TMEM87A Polyclonal antibody (25091-1-AP)

    IHC staining of human prostate cancer using 25091-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TMEM87A Polyclonal antibody (25091-1-AP)

    IHC staining of human prostate cancer using 25091-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inRAW 264.7 cells, MDA-MB-453s cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25091-1-AP targets TMEM87A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM87A fusion protein Ag18715 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 87A
Calculated molecular weight 555 aa, 63 kDa
Observed molecular weight 63-70 kDa
GenBank accession numberBC005335
Gene symbol TMEM87A
Gene ID (NCBI) 25963
RRIDAB_2879892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM87A antibody 25091-1-APDownload protocol
IHC protocol for TMEM87A antibody 25091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (12-16-2021)

This antibody recognized a band around 60 kd, however, this band is not sensitive to deglycosylation. What's worse, this band also occurred in solo loading buffer.

  • Applications: Western Blot