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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM9B Polyclonal antibody

TMEM9B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24331-1-AP

Synonyms

C11orf15, TMEM9 domain family, member B, TMEM9B, Transmembrane protein 9B

Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using TMEM9B Polyclonal antibody (24331-1-AP)

    WB analysis of rat liver using 24331-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 24331-1-AP (TMEM9B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using TMEM9B Polyclonal antibody (24331-1-AP)

    WB analysis of mouse colon using 24331-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 24331-1-AP (TMEM9B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TMEM9B Polyclonal antibody (24331-1-AP)

    IHC staining of human colon cancer using 24331-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 24331-1-AP (TMEM9B Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TMEM9B Polyclonal antibody (24331-1-AP)

    IHC staining of human colon cancer using 24331-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 24331-1-AP (TMEM9B Antibody) at dilution of 1:50.

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Tested Applications

Positive WB detected inrat liver tissue, mouse colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24331-1-AP is TMEM9B Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM9B fusion protein Ag19383 相同性解析による交差性が予測される生物種
Full Name TMEM9 domain family, member B
Calculated molecular weight 198 aa, 23 kDa
Observed molecular weight 23 kDa, 35 kDa
GenBank accession numberBC040124
Gene symbol TMEM9B
Gene ID (NCBI) 56674
RRIDAB_2879497
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM9B, also termed as Transmembrane protein 9B encodes a 198 amino-acidprotein that contains an N-terminal signal peptide, a single transmembrane region, three potential N-glycosylation sites, and three conserved cys-rich domains in the N-terminus. There is a dimer form of TMEM9B protein (PMID: 12359240). TMEM9B mRNA is expressed in a wide range of tissues and cells. TMEM9B is a lysosomal transmembrane protein that regulates the activity of inflammatory signaling pathways.

Protocols

Product Specific Protocols
WB protocol for TMEM9B antibody 24331-1-APDownload protocol
IHC protocol for TMEM9B antibody 24331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols