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  • Featured Product
  • KD/KO Validated

TMF1 Monoclonal antibody, PBS Only

TMF1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G12F2

Cat no : 67505-1-PBS

Synonyms

TMF, TATA element modulatory factor, ARA160, Androgen receptor-associated protein of 160 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMF1 Monoclonal antibody (67505-1-Ig)

    WB analysis using 67505-1-Ig (same clone as 67505-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67505-1-Ig (TMF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TMF1 Monoclonal antibody (67505-1-Ig)

    WB analysis of HeLa using 67505-1-Ig (same clone as 67505-1-PBS)

    WB result of TMF1 antibody (67505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMF1 transfected HeLa cells. This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMF1 Monoclonal antibody (67505-1-Ig)

    IF Staining of HepG2 using 67505-1-Ig (same clone as 67505-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMF1 antibody (67505-1-Ig, Clone: 3G12F2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67505-1-PBS targets TMF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMF1 fusion protein Ag17666 相同性解析による交差性が予測される生物種
Full Name TATA element modulatory factor 1
Calculated molecular weight 1093 aa, 123 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberBC126123
Gene symbol TMF1
Gene ID (NCBI) 7110
RRIDAB_2882728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.