Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, Daudi cells, HUVEC cells, Raji cells |
| Positive IHC detected in | mouse kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
27174-1-AP targets TMIGD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TMIGD1 fusion protein Ag25691 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane and immunoglobulin domain containing 1 |
| Calculated molecular weight | 262 aa, 29 kDa |
| Observed molecular weight | 45 kDa, 29 kDa |
| GenBank accession number | BC137201 |
| Gene Symbol | TMIGD1 |
| Gene ID (NCBI) | 388364 |
| RRID | AB_2880786 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UXZ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMIGD1 (Transmembrane and immunoglobulin domain containing 1) is involved in various cellular processes such as regulation of cell structure, cell-cell adhesion, and cellular movement. It protects cells from oxidative- and nutrient-deprivation-induced cell injury by stabilizing the transepithelial electric resistance and permeability of renal epithelial cells. TMIGD1 has a calculated molecular mass of 29 kDa, and can be detected as 45 kDa due to the N-glycosylation (PMID: 26342724).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMIGD1 antibody 27174-1-AP | Download protocol |
| IHC protocol for TMIGD1 antibody 27174-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Signal Intestinal brush border formation requires a TMIGD1-based intermicrovillar adhesion complex | ||
Int Immunopharmacol Macrophage-derived exosomes promote intestinal mucosal barrier dysfunction in inflammatory bowel disease by regulating TMIGD1 via mircroRNA-223 |