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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using TMLHE Polyclonal antibody (16621-1-AP)

    WB analysis of human liver using 16621-1-AP (same clone as 16621-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 16621-1-AP (TMLHE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMLHE Polyclonal antibody (16621-1-AP)

    WB analysis using 16621-1-AP (same clone as 16621-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 16621-1-AP (TMLHE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TMLHE Polyclonal antibody (16621-1-AP)

    WB analysis of Jurkat using 16621-1-AP (same clone as 16621-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16621-1-AP (TMLHE antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using TMLHE Polyclonal antibody (16621-1-AP)

    IHC staining of human stomach using 16621-1-AP (same clone as 16621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16621-1-AP (TMLHE antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16621-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TMLHE Polyclonal antibody, PBS Only

TMLHE Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16621-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

TMLD, TML-alpha-ketoglutarate dioxygenase, Epsilon-trimethyllysine hydroxylase, Epsilon-trimethyllysine 2-oxoglutarate dioxygenase, EC:1.14.11.8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16621-1-PBS targets TMLHE in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMLHE fusion protein Ag9919 相同性解析による交差性が予測される生物種
Full Name trimethyllysine hydroxylase, epsilon
Calculated molecular weight 376 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC025269
Gene Symbol TMLHE
Gene ID (NCBI) 55217
RRIDAB_2205303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVH6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Trimethyllysine dioxygenase (TMLHE) encodes the first enzyme in carnitine biosynthesis, N6-trimethyllysine dioxygenase (TMLD) and enzyme deficiency was suggested to be a risk factor for ASD.