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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMOD2 Monoclonal antibody

TMOD2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, chicken

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E7D1

Cat no : 68039-1-Ig

Synonyms

N TMOD, Neuronal tropomodulin, NTMOD, TMOD2, Tropomodulin 2, tropomodulin 2 (neuronal)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMOD2 Monoclonal antibody (68039-1-Ig)

    WB analysis using 68039-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68039-1-Ig (TMOD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TMOD2 Monoclonal antibody (68039-1-Ig)

    IHC staining of human gliomas using 68039-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68039-1-Ig (TMOD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inpig brain tissue, rabbit brain, chicken brain, pig cerebellum, rabbit cerebellum, chicken cerebellum
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68039-1-Ig targets TMOD2 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rabbit, chicken samples.

Tested Reactivity Human, pig, rabbit, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMOD2 fusion protein Ag7262 相同性解析による交差性が予測される生物種
Full Name tropomodulin 2 (neuronal)
Calculated molecular weight 39 kDa
Observed molecular weight39-40 kDa
GenBank accession numberBC064961
Gene symbol TMOD2
Gene ID (NCBI) 29767
RRIDAB_2918781
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMOD2 antibody 68039-1-IgDownload protocol
IHC protocol for TMOD2 antibody 68039-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols