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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMOD3 Polyclonal antibody

TMOD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12521-1-AP

Synonyms

TMOD3, Tropomodulin 3, tropomodulin 3 (ubiquitous), U Tmod, Ubiquitous tropomodulin, UTMOD

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TMOD3 Polyclonal antibody (12521-1-AP)

    WB analysis of Jurkat using 12521-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using TMOD3 Polyclonal antibody (12521-1-AP)

    WB analysis of human heart using 12521-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using TMOD3 Polyclonal antibody (12521-1-AP)

    WB analysis of human kidney using 12521-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using TMOD3 Polyclonal antibody (12521-1-AP)

    WB analysis of human lung using 12521-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TMOD3 Polyclonal antibody (12521-1-AP)

    WB analysis of mouse brain using 12521-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using TMOD3 Polyclonal antibody (12521-1-AP)

    WB analysis of mouse kidney using 12521-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using TMOD3 Polyclonal antibody (12521-1-AP)

    IP experiment of Jurkat using 12521-1-AP

    IP result of anti-TMOD3 (IP:12521-1-AP, 4ug; Detection:12521-1-AP 1:700) with Jurkat cells lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TMOD3 Polyclonal antibody (12521-1-AP)

    IHC staining of human kidney using 12521-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TMOD3 Polyclonal antibody (12521-1-AP)

    IHC staining of mouse heart using 12521-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using TMOD3 Polyclonal antibody (12521-1-AP)

    IHC staining of human colon using 12521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, human heart tissue, human kidney tissue, human lung tissue, mouse brain tissue, mouse kidney tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman kidney tissue, human colon tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12521-1-AP targets TMOD3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMOD3 fusion protein Ag3215 相同性解析による交差性が予測される生物種
Full Name tropomodulin 3 (ubiquitous)
Calculated molecular weight 352 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC020542
Gene symbol TMOD3
Gene ID (NCBI) 29766
RRIDAB_2205324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMOD3 antibody 12521-1-APDownload protocol
IHC protocol for TMOD3 antibody 12521-1-APDownload protocol
IP protocol for TMOD3 antibody 12521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Carcinogenesis

MiR-145 modulates the radiosensitivity of non-small cell lung cancer cells by suppression of TMOD3.

Authors - Hang Li
View Article
humanIHC

Comb Chem High Throughput Screen

Clinicopathological Characteristics, Prognosis, and Correlated Tumor Cell Function of Tropomodulin-3 in Pancreatic Adenocarcinoma

Authors - Bin Zhong
View Article