Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of Jurkat using 12521-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 12521-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12521-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12521-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 12521-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12521-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 12521-1-AP
IP result of anti-TMOD3 (IP:12521-1-AP, 4ug; Detection:12521-1-AP 1:700) with Jurkat cells lysate 4000 ug.
IHC staining of human kidney using 12521-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 12521-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 12521-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMOD3 Polyclonal antibody

TMOD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12521-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

TMOD3, Tropomodulin 3, tropomodulin 3 (ubiquitous), U Tmod, Ubiquitous tropomodulin, UTMOD

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, human heart tissue, human kidney tissue, human lung tissue, mouse brain tissue, mouse kidney tissue
Positive IP detected in	Jurkat cells
Positive IHC detected in	human kidney tissue, human colon tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

12521-1-AP targets TMOD3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TMOD3 fusion protein Ag3215 相同性解析による交差性が予測される生物種
Full Name	tropomodulin 3 (ubiquitous)
Calculated molecular weight	352 aa, 40 kDa
Observed molecular weight	40 kDa
GenBank accession number	BC020542
Gene Symbol	TMOD3
Gene ID (NCBI)	29766
RRID	AB_2205324
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NYL9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TMOD3 antibody 12521-1-AP	Download protocol
IHC protocol for TMOD3 antibody 12521-1-AP	Download protocol
IP protocol for TMOD3 antibody 12521-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Carcinogenesis MiR-145 modulates the radiosensitivity of non-small cell lung cancer cells by suppression of TMOD3. Authors - Hang Li View Article
human	IHC	Comb Chem High Throughput Screen Clinicopathological Characteristics, Prognosis, and Correlated Tumor Cell Function of Tropomodulin-3 in Pancreatic Adenocarcinoma Authors - Bin Zhong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 12521-1-AP

WB analysis of human heart using 12521-1-AP

WB analysis of mouse brain using 12521-1-AP

WB analysis of human kidney using 12521-1-AP

WB analysis of human lung using 12521-1-AP

WB analysis of mouse kidney using 12521-1-AP

IP experiment of Jurkat using 12521-1-AP

IHC staining of human kidney using 12521-1-AP

IHC staining of mouse heart using 12521-1-AP

IHC staining of human colon using 12521-1-AP