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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TMOD4 Polyclonal antibody (11753-1-AP)

    WB analysis of mouse skeletal muscle using 11753-1-AP (same clone as 11753-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11753-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TMOD4 Polyclonal antibody (11753-1-AP)

    WB analysis of HeLa using 11753-1-AP (same clone as 11753-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11753-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using TMOD4 Polyclonal antibody (11753-1-AP)

    WB analysis of human brain using 11753-1-AP (same clone as 11753-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11753-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using TMOD4 Polyclonal antibody (11753-1-AP)

    IP experiment of mouse skeletal muscle using 11753-1-AP (same clone as 11753-1-PBS)

    IP result of anti-TMOD4 (IP:11753-1-AP, 4ug; Detection:11753-1-AP 1:600) with mouse skeletal muscle tissue lysate 3600ug. This data was developed using the same antibody clone with 11753-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TMOD4 Polyclonal antibody (11753-1-AP)

    IHC staining of human gliomas using 11753-1-AP (same clone as 11753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11753-1-AP (TMOD4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11753-1-PBS in a different storage buffer formulation.

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TMOD4 Polyclonal antibody, PBS Only

TMOD4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11753-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11753-1-PBS targets TMOD4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMOD4 fusion protein Ag2349 相同性解析による交差性が予測される生物種
Full Name tropomodulin 4 (muscle)
Calculated molecular weight 345 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC017810
Gene Symbol TMOD4
Gene ID (NCBI) 29765
RRIDAB_2205433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZQ9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TMOD4 is a member of tropomodulin (Tmod) family proteins which are capping proteins binding to the pointed end of actin filaments and preventing both elongation and depolymerization. Four mammalian Tmod genes have been identified to date. Tomd1 is expressed in terminally-differentiated post-mitotic cells, like heart, brain, lens, vasculature, and erythrocytes; Tmod2 expressed in neurons; Tmod3 expressed ubiquitously; Tmod4 expressed only in skeletal muscles. This antibody was raised against the full-length human TMOD4 and may cross-react with other Tmods.