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TMPO/LAP2 Polyclonal antibody

Name: TMPO/LAP2 Polyclonal antibody
Brand: Proteintech
SKU: 14651-1-AP
Rating: 4.00 (1 reviews)

TMPO/LAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 14651-1-AP

Synonyms

LAP2, TMPO, Lamina-associated polypeptide 2, isoforms beta/gamma, Thymopoietin-related peptide isoforms beta/gamma, Thymopoietin isoform alpha

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 14651-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14651-1-AP
WB result of TMPO/LAP2 antibody (14651-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMPO/LAP2 transfected HeLa cells.
IP experiment of HeLa using 14651-1-AP
IP result of anti-TMPO/LAP2 (IP:14651-1-AP, 4ug; Detection:14651-1-AP 1:500) with HeLa cells lysate 1600ug.
IHC staining of human breast cancer using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 14651-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14651-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (14651-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa cells using 14651-1-AP
IF result of anti-TMPO (14651-1-AP) in Hela cell by Dr. Ralph Kehlenbach.

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Cat No. 14651-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HSC-T6 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human liver tissue, mouse lung tissue, human liver cancer tissue, mouse brain tissue, rat brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 4 publications below
IF	See 4 publications below

Product Information

14651-1-AP targets TMPO/LAP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TMPO/LAP2 fusion protein Ag6247 相同性解析による交差性が予測される生物種
Full Name	thymopoietin
Calculated molecular weight	51 kDa
Observed molecular weight	75 kDa, 51 kDa and 39 kDa
GenBank accession number	BC053675
Gene symbol	TMPO/LAP2
Gene ID (NCBI)	7112
RRID	AB_2205440
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).

Protocols

Product Specific Protocols
WB protocol for TMPO/LAP2 antibody 14651-1-AP	Download protocol
IHC protocol for TMPO/LAP2 antibody 14651-1-AP	Download protocol
IF protocol for TMPO/LAP2 antibody 14651-1-AP	Download protocol
IP protocol for TMPO/LAP2 antibody 14651-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse		Nat Commun The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus. Authors - Ying Zhang View Article
human	IF	J Cell Biol A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies. Authors - Tino Pleiner View Article
human	WB	Sci Rep MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells. Authors - Yuhua Fu View Article
bovine	WB	J Dairy Sci Anti-inflammation of epicatechin mediated by TMEM35A and TMPO in bovine mammary epithelial cell line cells and mouse mammary gland. Authors - Xiao Ma KO Validated View Article
human	WB	World J Surg Oncol Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells. Authors - Lin Zhang KD Validated View Article
mouse	IF	Theranostics Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane. Authors - Xiaolong Xu View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Lauren (Verified Customer) (02-20-2020)

Tested fixes include -20'C Methanol for 10 mins, and both 2% and 4% Formaldehyde for 20 mins. We saw a good signal in all three fixes.

Applications: Immunofluorescence,
Primary Antibody Dilution: 1:100
Cell Tissue Type: mIMCD3

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMPO/LAP2 Polyclonal antibody

TMPO/LAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14651-1-AP

WB analysis of HeLa using 14651-1-AP

IP experiment of HeLa using 14651-1-AP

IHC staining of human breast cancer using 14651-1-AP

IHC staining of human breast cancer using 14651-1-AP

IHC staining of human liver using 14651-1-AP

IHC staining of human liver using 14651-1-AP

IHC staining of human liver cancer using 14651-1-AP

IHC staining of human liver cancer using 14651-1-AP

IHC staining of mouse brain using 14651-1-AP

IHC staining of mouse brain using 14651-1-AP

IHC staining of rat brain using 14651-1-AP

IHC staining of rat brain using 14651-1-AP

IHC staining of mouse lung using 14651-1-AP

IHC staining of mouse lung using 14651-1-AP

IHC staining of mouse lung using 14651-1-AP

IF Staining of HeLa using 14651-1-AP

IF Staining of HeLa cells using 14651-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.

A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies.

MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells.

Anti-inflammation of epicatechin mediated by TMEM35A and TMPO in bovine mammary epithelial cell line cells and mouse mammary gland.

Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells.

Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane.

Reviews