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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMPPE Recombinant antibody

TMPPE Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241180C8

Cat no : 84028-2-RR

Synonyms

241180C8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMPPE Recombinant antibody (84028-2-RR)

    WB analysis using 84028-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84028-2-RR (TMPPE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84028-2-RR

    Affinity and Kinetic Characterization of 84028-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84028-2-RR against Human TMPPE were performed. The affinity constant is 82.2 pM.

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293T cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84028-2-RR is TMPPE Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMPPE fusion protein Ag19556 相同性解析による交差性が予測される生物種
Full Name transmembrane protein with metallophosphoesterase domain
Calculated molecular weight 453 aa, 49 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC136873
Gene symbol TMPPE
Gene ID (NCBI) 643853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMPPE antibody 84028-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols