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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMX4 Polyclonal antibody

TMX4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21348-1-AP

Synonyms

UNQ475/PRO938, TXNDC13, Thioredoxin-related transmembrane protein 4, Thioredoxin domain-containing protein 13, PDIA14

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using TMX4 Polyclonal antibody (21348-1-AP)

    WB analysis of mouse brain using 21348-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TMX4 Polyclonal antibody (21348-1-AP)

    WB analysis of mouse brain using 21348-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TMX4 Polyclonal antibody (21348-1-AP)

    WB analysis of mouse brain using 21348-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using TMX4 Polyclonal antibody (21348-1-AP)

    WB analysis of human heart using 21348-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using TMX4 Polyclonal antibody (21348-1-AP)

    WB analysis of L02 using 21348-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using TMX4 Polyclonal antibody (21348-1-AP)

    WB analysis of A375 using 21348-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using TMX4 Polyclonal antibody (21348-1-AP)

    IHC staining of human brain using 21348-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using TMX4 Polyclonal antibody (21348-1-AP)

    IHC staining of human brain using 21348-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TMX4 Polyclonal antibody (21348-1-AP)

    IHC staining of human testis using 21348-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TMX4 Polyclonal antibody (21348-1-AP)

    IHC staining of human testis using 21348-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TMX4 Polyclonal antibody (21348-1-AP)

    IHC staining of human testis using 21348-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TMX4 Polyclonal antibody (21348-1-AP)

    IHC staining of human testis using 21348-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMX4 Polyclonal antibody (21348-1-AP)

    IF Staining of HepG2 using 21348-1-AP

    Immunofluorescent analysis of HepG2 cells using 21348-1-AP (TMX4 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse brain tissue, A375 cells, human heart tissue, L02 cells
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21348-1-AP targets TMX4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMX4 fusion protein Ag15696 相同性解析による交差性が予測される生物種
Full Name thioredoxin-related transmembrane protein 4
Calculated molecular weight 349 aa, 39 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC033787
Gene symbol TMX4
Gene ID (NCBI) 56255
RRIDAB_10733353
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMX4(Thioredoxin-related transmembrane protein 4) is also named as KIAA1162, TXNDC13. It is a type I transmembrane protein, with a Trx-like domain facing the ER lumen and may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER. It could work as a reductase in the environment of the ER(PMID: 20056998). The full length protein has a 23 amino acid signal peptide. This antibody is specific to TMX4.

Protocols

Product Specific Protocols
WB protocol for TMX4 antibody 21348-1-APDownload protocol
IHC protocol for TMX4 antibody 21348-1-APDownload protocol
IF protocol for TMX4 antibody 21348-1-APDownload protocol
FC protocol for TMX4 antibody 21348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IF

Nat Commun

TMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress

Authors - Marika K Kucińska
View Article
humanWB

J Proteome Res

Mapping of the N-linked glycoproteome of human spermatozoa.

Authors - Wang Gaigai G
View Article
mouseWB

JCI Insight

Protein disulfide isomerase is essential for spermatogenesis in mice

Authors - Yaqiong Zhang
View Article