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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNC/Tenascin-C Monoclonal antibody, PBS Only

TNC/Tenascin-C Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7G8

Cat no : 67710-1-PBS

Synonyms

Cytotactin, GMEM, GP 150 225, Hexabrachion, HXB, JI, Myotendinous antigen, Neuronectin, Tenascin, tenascin C, TN, TN C, TNC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    WB analysis using 67710-1-Ig (same clone as 67710-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    WB analysis of rat cerebellum using 67710-1-Ig (same clone as 67710-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    WB analysis of HEK-293 using 67710-1-Ig (same clone as 67710-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    IHC staining of human breast cancer using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    IHC staining of human breast cancer using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    IHC staining of rat brain using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    IHC staining of rat brain using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    IHC staining of human liver cancer using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    IHC staining of human liver cancer using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    IHC staining of human lung cancer using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    IHC staining of human lung cancer using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    IHC staining of mouse brain using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    IHC staining of mouse brain using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using TNC/Tenascin-C Monoclonal antibody (67710-1-Ig)

    IF Staining of rat brain using 67710-1-Ig (same clone as 67710-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TNC/Tenascin-C antibody (67710-1-Ig, Clone: 1F7G8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67710-1-PBS targets TNC/Tenascin-C in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TNC/Tenascin-C fusion protein Ag21412 相同性解析による交差性が予測される生物種
Full Name tenascin C
Calculated molecular weight 2201 aa, 241 kDa
Observed molecular weight260 kDa
GenBank accession numberBC151843
Gene symbol TNC
Gene ID (NCBI) 3371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.