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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNF-alpha Monoclonal antibody (60291-1-Ig)

    WB analysis using 60291-1-Ig (same clone as 60291-1-PBS)

    PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 60291-1-Ig (TNF Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody 60004-1-Ig as loading control. This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TNF-alpha Monoclonal antibody (60291-1-Ig)

    WB analysis of HeLa using 60291-1-Ig (same clone as 60291-1-PBS)

    WB result of TNF alpha antibody (60291-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TNF alpha transfected HeLa cells. This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TNF-alpha Monoclonal antibody (60291-1-Ig)

    IHC staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TNF-alpha Monoclonal antibody (60291-1-Ig)

    IHC staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TNF-alpha Monoclonal antibody (60291-1-Ig)

    IHC staining of human tonsillitis using 60291-1-Ig (same clone as 60291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TNF-alpha Monoclonal antibody (60291-1-Ig)

    IHC staining of human tonsillitis using 60291-1-Ig (same clone as 60291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60291-1-Ig (TNF Alpha antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using TNF-alpha Monoclonal antibody (60291-1-Ig)

    IF Staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using TNF-alpha Monoclonal antibody (60291-1-Ig)

    IF Staining of human breast cancer using 60291-1-Ig (same clone as 60291-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60291-1-Ig (TNF alpha antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using TNF-alpha Monoclonal antibody (60291-1-Ig)

    IF Staining of THP-1 using 60291-1-Ig (same clone as 60291-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PMA, LPS and Brefeldin A treated THP-1 cells using TNF-alpha antibody (60291-1-Ig, Clone: 7B8A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60291-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60291-1-PBS targets TNF-alpha in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TNF-alpha fusion protein Ag11413 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight26 kDa
GenBank accession numberBC028148
Gene Symbol TNF-alpha
Gene ID (NCBI) 7124
ENSEMBL Gene IDENSG00000232810
RRIDAB_2833255
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01375
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.