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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNF beta Polyclonal antibody

TNF beta Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31505-1-AP

Synonyms

LTA, LT alpha, TNFB, TNF β, Lymphotoxin-alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNF Beta Polyclonal antibody (31505-1-AP)

    WB analysis using 31505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31505-1-AP (TNF Beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using TNF Beta Polyclonal antibody (31505-1-AP)

    WB analysis of mouse spleen using 31505-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 31505-1-AP (TNF Beta antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using TNF Beta Polyclonal antibody (31505-1-AP)

    WB analysis of rat spleen using 31505-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 31505-1-AP (TNF Beta antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31505-1-AP targets TNF beta in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name lymphotoxin alpha (TNF superfamily, member 1)
Calculated molecular weight 205 aa, 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC034729
Gene symbol TNF beta
Gene ID (NCBI) 4049
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNF beta also known as lymphotoxin alpha (LTA), is a member of the tumor necrosis factor family, and is a cytokine produced by lymphocytes. TNF Beta is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. TNF Beta also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

Protocols

Product Specific Protocols
WB protocol for TNF beta antibody 31505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols