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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNF-alpha Recombinant antibody, PBS Only (Detector)

TNF-alpha Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240668A4

Cat no : 83721-6-PBS

Synonyms

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

Validation Data Gallery

Filter:
  • Sandwich ELISA standard curve of MP00702-4

    Sandwich ELISA standard curve of MP00702-4

    Sandwich ELISA standard curve of MP00702-4, TNF-alpha Recombinant Matched Antibody Pair - PBS only. 83721-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0816. 83721-6-PBS was HRP conjugated as the detection antibody. Range: 3.9-250 pg/mL.

  • Sample test of MP00702-4

    Sample test of MP00702-4

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean TNF-alpha concentration was determined to be 1.3 pg/mL in unstimulated PBMC supernatant,1236.3 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP00702-4

    Sample test of MP00702-4

    Serum of seven individual healthy human donors was measured. The human TNF-alpha concentration of detected samples was determined to be 3.25 pg/mL with a range of ND-9.1 pg/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83721-6-PBS targets TNF-alpha as part of a matched antibody pair:

MP00702-4: 83721-5-PBS capture and 83721-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated molecular weight26 kDa
GenBank accession numberNM_000594.4
Gene symbol TNF-alpha
Gene ID (NCBI) 7124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.