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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human TNF-alpha Rabbit Recombinant Antibody, PBS Only

TNF-alpha Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240668B11

Cat no : 98064-1-PBS

Synonyms

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TNF-alpha Rabbit Recombinant Antibody (98064-1-RR)

    FC experiment of human PBMCs using 98064-1-RR (same clone as 98064-1-PBS)

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNF-alpha Rabbit Recombinant Antibody (98064-1-RR, Clone:240668B11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 98064-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TNF-alpha Rabbit Recombinant Antibody (98064-1-RR)

    FC experiment of human PBMCs using 98064-1-RR (same clone as 98064-1-PBS)

    1x10^6 untreated(black) or PMA, Ionomycin and Brefeldin A treated(red) human PBMCs were intracellularly stained with 0.25 ug Anti-Human TNF-alpha Rabbit Recombinant Antibody (98064-1-RR, Clone:240668B11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 98064-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98064-1-RR

    Affinity and Kinetic Characterization of 98064-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98064-1-RR against Human TNF-alpha were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98064-1-PBS is TNF-alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated molecular weight26 kDa
GenBank accession numberNM_000594.4
Gene symbol TNF-alpha
Gene ID (NCBI) 7124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.