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  • Featured Product
  • KD/KO Validated

TNFAIP1 Polyclonal antibody

TNFAIP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15320-1-AP

Synonyms

Tumor necrosis factor, alpha-induced protein 1, endothelial, EDP1, BTB/POZ domain-containing protein TNFAIP1, BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using TNFAIP1 Polyclonal antibody (15320-1-AP)

    WB analysis of HeLa using 15320-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15320-1-AP (TNFAIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using TNFAIP1 Polyclonal antibody (15320-1-AP)

    WB analysis of L02 using 15320-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15320-1-AP (TNFAIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TNFAIP1 Polyclonal antibody (15320-1-AP)

    IHC staining of human placenta using 15320-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TNFAIP1 Polyclonal antibody (15320-1-AP)

    IHC staining of human placenta using 15320-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using TNFAIP1 Polyclonal antibody (15320-1-AP)

    IHC staining of human brain using 15320-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using TNFAIP1 Polyclonal antibody (15320-1-AP)

    IHC staining of human brain using 15320-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TNFAIP1 Polyclonal antibody (15320-1-AP)

    IF Staining of HeLa using 15320-1-AP

    Immunofluorescent analysis of HeLa cells using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman placenta tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15320-1-AP targets TNFAIP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFAIP1 fusion protein Ag0220 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor, alpha-induced protein 1 (endothelial)
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC001949
Gene symbol TNFAIP1
Gene ID (NCBI) 7126
RRIDAB_10644288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNFAIP1 was identified as a human gene activated transcriptionally by tumour necrosis factor alpha in endothelial cells. It is encoded by a 3.5-kilobase transcript and its expression is found to be developmentally regulated in a tissue-specific manner. The open reading frame predicts a 316-amino acid sequence rich in charged residues, particularly at the carboxyl terminus.

Protocols

Product Specific Protocols
WB protocol for TNFAIP1 antibody 15320-1-APDownload protocol
IHC protocol for TNFAIP1 antibody 15320-1-APDownload protocol
IF protocol for TNFAIP1 antibody 15320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Commun Signal

Neuronal-specific TNFAIP1 ablation attenuates postoperative cognitive dysfunction via targeting SNAP25 for K48-linked ubiquitination

Authors - Wei Wang
View Article
mouseWB

Neurotox Res

TNFAIP1 Mediates Formaldehyde-Induced Neurotoxicity by Inhibiting the Akt/CREB Pathway in N2a Cells.

Authors - Junzhi Yi
  • KD Validated
View Article
humanWB

Oncol Lett

MicroRNA-373 is upregulated and targets TNFAIP1 in human gastric cancer, contributing to tumorigenesis.

Authors - Zhang Xiaoting X
  • KD Validated
View Article