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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    WB analysis using 66695-1-Ig (same clone as 66695-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    WB analysis of HSC-T6 using 66695-1-Ig (same clone as 66695-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    IHC staining of rat kidney using 66695-1-Ig (same clone as 66695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    IHC staining of mouse kidney using 66695-1-Ig (same clone as 66695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    IHC staining of mouse kidney using 66695-1-Ig (same clone as 66695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    IHC staining of rat kidney using 66695-1-Ig (same clone as 66695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat ovary tissue using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    IHC staining of rat ovary using 66695-1-Ig (same clone as 66695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    IHC staining of human thyroid cancer using 66695-1-Ig (same clone as 66695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    IHC staining of human thyroid cancer using 66695-1-Ig (same clone as 66695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat ovary tissue using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    IHC staining of rat ovary using 66695-1-Ig (same clone as 66695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    IF Staining of HeLa using 66695-1-Ig (same clone as 66695-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TNFAIP3 antibody (66695-1-Ig, Clone: 3A11G6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TNFAIP3 Monoclonal antibody (66695-1-Ig)

    FC experiment of HeLa using 66695-1-Ig (same clone as 66695-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human TNFAIP3 (66695-1-Ig, Clone:3A11G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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TNFAIP3 Monoclonal antibody, PBS Only

TNFAIP3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66695-1-PBS
Clone No.3A11G6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

A20, A20p37, A20p50, EC:2.3.2.-, OTU domain-containing protein 7C

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66695-1-PBS targets TNFAIP3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TNFAIP3 fusion protein Ag18150 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor, alpha-induced protein 3
Calculated molecular weight 790 aa, 90 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC114480
Gene Symbol TNFAIP3
Gene ID (NCBI) 7128
RRIDAB_2882048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21580
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.