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KD/KO Validated

TSG-6 Polyclonal antibody

Name: TSG-6 Polyclonal antibody
Brand: Proteintech
SKU: 13321-1-AP

TSG-6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13321-1-AP

Synonyms

Hyaluronate binding protein, TNF alpha induced protein 6, TNF stimulated gene 6 protein, TNFAIP6, TSG 6, TSG6, TSG-6

Validation Data Gallery

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AllWBIHC

WB analysis of NIH/3T3 using 13321-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13321-1-AP (TSG-6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13321-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13321-1-AP (TSG-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of rat kidney using 13321-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 13321-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 13321-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 13321-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 13321-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 13321-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 13321-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	NIH/3T3 cells, A549 cells
Positive IHC detected in	rat kidney tissue, human cervical cancer tissue, human ovary tumor tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 3 publications below
IF	See 2 publications below

Product Information

13321-1-AP targets TSG-6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TSG-6 fusion protein Ag3885 相同性解析による交差性が予測される生物種
Full Name	tumor necrosis factor, alpha-induced protein 6
Calculated molecular weight	277 aa, 31 kDa
Observed molecular weight	39 kDa
GenBank accession number	BC030205
Gene symbol	TNFAIP6
Gene ID (NCBI)	7130
RRID	AB_2204641
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TSG-6 antibody 13321-1-AP	Download protocol
IHC protocol for TSG-6 antibody 13321-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	JACC Basic Transl Sci Atheroprotective Effects of Tumor Necrosis Factor-Stimulated Gene-6. Authors - Rena Watanabe View Article
mouse	WB	Front Pharmacol TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis via Suppression of STAT3 Activation. Authors - Yue-Meng Wan View Article
rat	WB	Neurochem Res Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Secreted by BMSCs Regulates Activated Astrocytes by Inhibiting NF-κB Signaling Pathway to Ameliorate Blood Brain Barrier Damage After Intracerebral Hemorrhage. Authors - Bin Tang View Article
human	WB,IHC	Contrast Media Mol Imaging TNFAIP6 Promotes Gastric Carcinoma Cell Invasion via Upregulating PTX3 and Activating the Wnt/β-Catenin Signaling Pathway Authors - Hongquan Cui KD Validated View Article
human	WB,IHC,IF	Neural Regen Res Tumor necrosis factor-stimulated gene-6 ameliorates early brain injury after subarachnoid hemorrhage by suppressing NLRC4 inflammasome-mediated astrocyte pyroptosis Authors - Mingxiang Ding View Article
human	WB,IHC	Front Immunol Neutrophil infiltration associated genes on the prognosis and tumor immune microenvironment of lung adenocarcinoma Authors - Renwang Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TSG-6 Polyclonal antibody

TSG-6 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of NIH/3T3 using 13321-1-AP

WB analysis of A549 using 13321-1-AP

IHC staining of rat kidney using 13321-1-AP

IHC staining of rat kidney using 13321-1-AP

IHC staining of human cervical cancer using 13321-1-AP

IHC staining of human ovary tumor using 13321-1-AP

IHC staining of mouse kidney using 13321-1-AP

IHC staining of mouse kidney using 13321-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Atheroprotective Effects of Tumor Necrosis Factor-Stimulated Gene-6.

TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis via Suppression of STAT3 Activation.

Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Secreted by BMSCs Regulates Activated Astrocytes by Inhibiting NF-κB Signaling Pathway to Ameliorate Blood Brain Barrier Damage After Intracerebral Hemorrhage.

TNFAIP6 Promotes Gastric Carcinoma Cell Invasion via Upregulating PTX3 and Activating the Wnt/β-Catenin Signaling Pathway

Tumor necrosis factor-stimulated gene-6 ameliorates early brain injury after subarachnoid hemorrhage by suppressing NLRC4 inflammasome-mediated astrocyte pyroptosis

Neutrophil infiltration associated genes on the prognosis and tumor immune microenvironment of lung adenocarcinoma