Validation Data Gallery
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Tested Applications
| Positive WB detected in | NIH/3T3 cells, A549 cells |
| Positive IHC detected in | rat kidney tissue, human cervical cancer tissue, human ovary tumor tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
13321-1-AP targets TSG-6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TSG-6 fusion protein Ag3885 相同性解析による交差性が予測される生物種 |
| Full Name | tumor necrosis factor, alpha-induced protein 6 |
| Calculated molecular weight | 277 aa, 31 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC030205 |
| Gene Symbol | TNFAIP6 |
| Gene ID (NCBI) | 7130 |
| RRID | AB_2204641 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98066 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TSG-6 antibody 13321-1-AP | Download protocol |
| IHC protocol for TSG-6 antibody 13321-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
JACC Basic Transl Sci Atheroprotective Effects of Tumor Necrosis Factor-Stimulated Gene-6. | ||
Front Pharmacol TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis via Suppression of STAT3 Activation. | ||
Neurochem Res Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Secreted by BMSCs Regulates Activated Astrocytes by Inhibiting NF-κB Signaling Pathway to Ameliorate Blood Brain Barrier Damage After Intracerebral Hemorrhage. | ||
Contrast Media Mol Imaging TNFAIP6 Promotes Gastric Carcinoma Cell Invasion via Upregulating PTX3 and Activating the Wnt/β-Catenin Signaling Pathway
| ||
Neural Regen Res Tumor necrosis factor-stimulated gene-6 ameliorates early brain injury after subarachnoid hemorrhage by suppressing NLRC4 inflammasome-mediated astrocyte pyroptosis | ||
Front Immunol Neutrophil infiltration associated genes on the prognosis and tumor immune microenvironment of lung adenocarcinoma |