Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, PC-13 cells, A549 cells, MOLT-4 cells |
| Positive IHC detected in | human lung cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 14 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
15790-1-AP targets TNFAIP8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TNFAIP8 fusion protein Ag8491 相同性解析による交差性が予測される生物種 |
| Full Name | tumor necrosis factor, alpha-induced protein 8 |
| Calculated molecular weight | 198 aa, 23 kDa |
| Observed molecular weight | 19-23 kDa |
| GenBank accession number | BC007014 |
| Gene Symbol | TNFAIP8 |
| Gene ID (NCBI) | 25816 |
| RRID | AB_10792805 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TNFAIP8 (tumor necrosis factor, alpha-induced protein 8), also called SCC-S2/GG2-1/NDED, is associated with enhanced cell survival and inhibition of apoptosis. The induction of TNFAIP8 by TNF depends on the activation of NFκB. TNFAIP8 suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. TNFAIP8 is expressed in adult spleen, lymph node, thymus, thyroid, bone marrow, and placenta, and in fetal liver, lung, and kidney at high levels, also expressed in various tumor tissues and all cancer cell lines tested. There are four major isoforms of TNFAIP8.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNFAIP8 antibody 15790-1-AP | Download protocol |
| IHC protocol for TNFAIP8 antibody 15790-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunol Crucial Roles of TNFAIP8 Protein in Regulating Apoptosis and Listeria Infection. | ||
Oncotarget TNFAIP8 promotes prostate cancer cell survival by inducing autophagy.
| ||
Toxins (Basel) Cyanotoxins Increase Cytotoxicity and Promote Nonalcoholic Fatty Liver Disease Progression by Enhancing Cell Steatosis | ||
J Proteome Res Proteomic Analysis of Plasma sEVs Reveals That TNFAIP8 Is a New Biomarker of Cell Proliferation in Diabetic Retinopathy.
| ||
Sci Rep The TIPE Molecular Pilot That Directs Lymphocyte Migration in Health and Inflammation.
| ||
Sci Rep The Tnfaip8-PE complex is a novel upstream effector in the anti-autophagic action of insulin.
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