Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 15940-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 15940-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 15940-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat lymph using 15940-1-AP
rat lymph tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 15940-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse spleen using 15940-1-AP
IP result of anti-TIPE2 (IP:15940-1-AP, 4ug; Detection:15940-1-AP 1:2000) with mouse spleen tissue lysate 1400 ug.
IHC staining of human lymphoma using 15940-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 15940-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 15940-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 15940-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 15940-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 15940-1-AP (TIPE2 antibody at dilution of 1:50.
IHC staining of human ovary tumor using 15940-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 15940-1-AP (TIPE2 antibody at dilution of 1:50.
IF Staining of RAW 264.7 using 15940-1-AP
Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIPE2 antibody (15940-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 15940-1-AP
Immunofluorescent analysis of HepG2 cells using 15940-1-AP (TIPE2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of THP-1 using 15940-1-AP
1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TIPE2 (15940-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

TNFAIP8L2 Polyclonal antibody

TNFAIP8L2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15940-1-AP

Publications(37)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

TIPE2, Inflammation factor protein 20, Tumor necrosis factor alpha-induced protein 8-like protein 2, TNFAIP8-like protein 2, TNFAIP8 like protein 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (37)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	THP-1 cells, mouse small intestine tissue, mouse cerebellum tissue, rat lymph tissue, rat spleen tissue, RAW 264.7 cells, mouse spleen tissue
Positive IP detected in	mouse spleen tissue
Positive IHC detected in	human lymphoma tissue, human ovary tumor tissue, mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	RAW 264.7 cells, HepG2 cells
Positive FC (Intra) detected in	THP-1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 10 publications below
WB	See 32 publications below
IHC	See 12 publications below
IF	See 11 publications below
IP	See 2 publications below

Product Information

15940-1-AP targets TNFAIP8L2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TNFAIP8L2 fusion protein Ag7964 相同性解析による交差性が予測される生物種
Full Name	tumor necrosis factor, alpha-induced protein 8-like 2
Calculated molecular weight	184 aa, 21 kDa
Observed molecular weight	18-20 kDa
GenBank accession number	BC063014
Gene Symbol	TIPE2
Gene ID (NCBI)	79626
RRID	AB_2204663
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6P589
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TIPE2 (TNF-α-inducible protein 8-like 2, or TNFAIP8L2), a negative immune regulator, is essential for maintaining immune homeostasis. TIPE2 shares considerable sequence homology with members of the tumor necrosis factor-α-inducible protein 8 (TNFAIP8) family, which are thought to regulate cellular and immune homeostasis. TIPE2 acts as a negative regulator of Toll-like receptor and T-cell receptor function and is preferentially expressed in lymphoid tissues. TIPE2 plays an essential role for a signal transduction pathway that links inflammatory immune response to specific conditions after cerebral ischemia

Protocols

Product Specific Protocols
WB protocol for TNFAIP8L2 antibody 15940-1-AP	Download protocol
IHC protocol for TNFAIP8L2 antibody 15940-1-AP	Download protocol
IF protocol for TNFAIP8L2 antibody 15940-1-AP	Download protocol
IP protocol for TNFAIP8L2 antibody 15940-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. Authors - Li Jianhua J View Article
mouse	WB,IF,IP	Nat Immunol Direction of leukocyte polarization and migration by the phosphoinositide-transfer protein TIPE2. Authors - Svetlana A Fayngerts KO Validated View Article
mouse,human	WB	Sci Adv TIPE2 is a checkpoint of natural killer cell maturation and antitumor immunity. Authors - Jiacheng Bi KO Validated View Article
mouse	WB	Autophagy TNFAIP8L2/TIPE2 impairs autolysosome reformation via modulating the RAC1-MTORC1 axis. Authors - Wen Li KO Validated View Article
mouse	WB	J Exp Med TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis. Authors - Dehong Yan KO Validated View Article
human,mouse	WB	Cancer Immunol Res Tumor Necrosis Factor-α-Induced Protein 8-Like 2 Fosters Tumor-Associated Microbiota to Promote the Development of Colorectal Cancer. Authors - Yunwei Lou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15940-1-AP

WB analysis of mouse cerebellum using 15940-1-AP

WB analysis of mouse small intestine using 15940-1-AP

WB analysis of rat lymph using 15940-1-AP

WB analysis of rat spleen using 15940-1-AP

IP experiment of mouse spleen using 15940-1-AP

IHC staining of human lymphoma using 15940-1-AP

IHC staining of human lymphoma using 15940-1-AP

IHC staining of mouse spleen using 15940-1-AP

IHC staining of mouse spleen using 15940-1-AP

IHC staining of human ovary tumor using 15940-1-AP

IHC staining of human ovary tumor using 15940-1-AP

IF Staining of RAW 264.7 using 15940-1-AP

IF Staining of HepG2 using 15940-1-AP

FC experiment of THP-1 using 15940-1-AP

TNFAIP8L2 Polyclonal antibody

TNFAIP8L2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Direction of leukocyte polarization and migration by the phosphoinositide-transfer protein TIPE2.

TIPE2 is a checkpoint of natural killer cell maturation and antitumor immunity.

TNFAIP8L2/TIPE2 impairs autolysosome reformation via modulating the RAC1-MTORC1 axis.

TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.

Tumor Necrosis Factor-α-Induced Protein 8-Like 2 Fosters Tumor-Associated Microbiota to Promote the Development of Colorectal Cancer.