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  • Featured Product
  • KD/KO Validated

TNFR1/CD120a Monoclonal antibody, PBS Only

TNFR1/CD120a Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A6E3

Cat no : 60192-1-PBS

Synonyms

TNFRSF1A, p60, p55 R, p55, FPF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFR1/CD120a Monoclonal antibody (60192-1-Ig)

    WB analysis using 60192-1-Ig (same clone as 60192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1/CD120a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TNFR1/CD120a Monoclonal antibody (60192-1-Ig)

    WB analysis using 60192-1-Ig (same clone as 60192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using TNFR1/CD120a Monoclonal antibody (60192-1-Ig)

    WB analysis of Daudi using 60192-1-Ig (same clone as 60192-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using TNFR1/CD120a Monoclonal antibody (60192-1-Ig)

    WB analysis of HL-60 using 60192-1-Ig (same clone as 60192-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TNFR1/CD120a Monoclonal antibody (60192-1-Ig)

    IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1/CD120a Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TNFR1/CD120a Monoclonal antibody (60192-1-Ig)

    IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TNFR1/CD120a Monoclonal antibody (60192-1-Ig)

    IHC staining of human colon cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TNFR1/CD120a Monoclonal antibody (60192-1-Ig)

    IHC staining of human colon cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using TNFR1/CD120a Monoclonal antibody (60192-1-Ig)

    IF Staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TNFR1/CD120a antibody (60192-1-Ig, Clone: 2A6E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60192-1-PBS targets TNFR1/CD120a in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TNFR1/CD120a fusion protein Ag16112 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 1A
Calculated molecular weight 455 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC010140
Gene symbol TNFR1
Gene ID (NCBI) 7132
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.