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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNFR1/CD120a Recombinant antibody, PBS Only

TNFR1/CD120a Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241336B4

Cat no : 84243-5-PBS

Synonyms

CD120a, TNFR1, TNFRSF1A, 241336B4, p60

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFR1/CD120a Recombinant antibody (84243-5-RR)

    WB analysis using 84243-5-RR (same clone as 84243-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84243-5-RR (TNFR1/CD120a antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84243-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TNFR1/CD120a Recombinant antibody (84243-5-RR)

    IHC staining of human colon cancer using 84243-5-RR (same clone as 84243-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84243-5-RR (TNFR1/CD120a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84243-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TNFR1/CD120a Recombinant antibody (84243-5-RR)

    IHC staining of human colon cancer using 84243-5-RR (same clone as 84243-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84243-5-RR (TNFR1/CD120a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84243-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84243-5-RR

    Affinity and Kinetic Characterization of 84243-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84243-5-RR against Human TNFR1/CD120a were performed. The affinity constant is 20.5 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84243-5-PBS targets TNFR1/CD120a in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 1A
Calculated molecular weight 455 aa, 50 kDa
Observed molecular weight55 kDa
GenBank accession numberBC010140
Gene symbol TNFR1
Gene ID (NCBI) 7132
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.