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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human TNFR1/CD120a Rabbit Recombinant Antibody, PBS Only

TNFR1/CD120a Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241337H6

Cat no : 98085-1-PBS

Synonyms

TNFRSF1A, p60, p55 R, p55, FPF

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human TNFR1/CD120a Rabbit Recombinant Antibod (98085-1-RR)

    FC experiment of human peripheral blood leukocytes using 98085-1-RR (same clone as 98085-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human TNFR1/CD120a Rabbit Recombinant Antibody (98085-1-RR, Clone:241337H6) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98085-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human TNFR1/CD120a Rabbit Recombinant Antibod (98085-1-RR)

    FC experiment of human peripheral blood leukocytes using 98085-1-RR (same clone as 98085-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human TNFR1/CD120a Rabbit Recombinant Antibody (98085-1-RR, Clone:241337H6) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98085-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human TNFR1/CD120a Rabbit Recombinant Antibod (98085-1-RR)

    FC experiment of human peripheral blood leukocytes using 98085-1-RR (same clone as 98085-1-PBS)

    1x10^6 FC receptor blocked human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human TNFR1/CD120a Rabbit Recombinant Antibody (98085-1-RR, Clone:241337H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98085-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98085-1-RR

    Affinity and Kinetic Characterization of 98085-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98085-1-RR against Human TNFR1/CD120a were performed. The affinity constant is 3.79 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98085-1-PBS targets TNFR1/CD120a in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 1A
Calculated molecular weight 455 aa, 50 kDa
GenBank accession numberBC010140
Gene symbol TNFR1
Gene ID (NCBI) 7132
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.