TNFR2 / TNFRSF1B Polyclonal antibody

TNFR2 / TNFRSF1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28746-1-AP

Synonyms

CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B


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Tested Applications

Positive WB detected inTHP-1 cells, U-937 cells
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28746-1-AP targets TNFR2 / TNFRSF1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFR2 / TNFRSF1B fusion protein Ag30490 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 1B
Calculated molecular weight 48 kDa
Observed molecular weight 75 kDa, 65 kDa
GenBank accession numberBC052977
Gene symbol TNFR2
Gene ID (NCBI) 7133
RRIDAB_2881205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).

Protocols

Product Specific Protocols
WB protocol for TNFR2 / TNFRSF1B antibody 28746-1-APDownload protocol
IHC protocol for TNFR2 / TNFRSF1B antibody 28746-1-APDownload protocol
IF protocol for TNFR2 / TNFRSF1B antibody 28746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure

Authors - Huilong Li
humanIHC

Oncol Lett

Association between tumor necrosis factor receptor 2 and progression and poor prognosis of tumor stage 2‑3 esophageal squamous cell carcinoma and stratified analysis

Authors - Zifeng Liu
humanIHC,IF

Front Immunol

Integrating machine learning algorithms and multiple immunohistochemistry validation to unveil novel diagnostic markers based on costimulatory molecules for predicting immune microenvironment status in triple-negative breast cancer

Authors - Chao Zhang
humanIF

Am J Pathol

TNF receptor-2 signals clear-cell renal carcinoma proliferation via phosphorylated-4EBP1 and mitochondrial gene translation

Authors - Rafia S Al-Lamki